Basic Research Plan
The following is a minimal research plan for a bacteria related project. Note, this project would require Form 2 (Qualified Scientist) and Form 6A (Potentially Hazardous Biological Agents) as well,
A. Problem: Do commercial cleaning products reduce the amount of bacteria on a surface?
B. Hyposesis: Commercial cleaning products kill a significant amount of bacteria
- 6 large, 10 cm (4 in) diameter Petri dishes
- 6 cotton swabs
- 5 grams of Agar
- 1 600 mL plastic beaker
- 2 types of cleaning products
- 1 incubator
- Choose two locations with sufficient space to take two samples after cleaning
- Prepare agar and place into petri dishes
- Repeat the next steps for both locations
- Transfer a bacteria sample using a cotton swap from the center of the area and swab it on a petri dish
- Clean half the area with a cleaning product and allow to dry. Get another sample into the next petri dish
- Repeat with the other cleaning product on the other half of the area.
- Seal the petri dishes
- Place petri dishes into incubator set to 35 degrees Celcius
- Allow bateria to grow for one week, record status daily measuring number, color and size of growths
- Clean up
- The cotton swabs will be discarded in the normal trash
- Petri dishes will be autoclaved when the experiment is complete
- Risk Assesment
- The cotton swabs only have the amount of bacteria the a person would normally be exposed to so they can be discarded after use
- Bacteria growth mush be done in a biosaftey level 1 location. Our school lab is BSL-1.
- The petri dishes have large amounts of bacteria and need to be disposed of properly. They will also remain sealed after the samples are obtained.
- Alcamo IE (2001). Fundamentals of microbiology. Boston: Jones and Bartlett. ISBN 0-7637-1067-9.
- Atlas RM (1995). Principles of microbiology. St. Louis: Mosby. ISBN 0-8016-7790-4.
- Bacteria, http://en.wikipedia.org/wiki/Bacteria
- Martinko JM, Madigan MT (2005). Brock Biology of Microorganisms (11th ed.). Englewood Cliffs, N.J: Prentice Hall. ISBN 0-13-144329-1.
- Funke BR, Tortora GJ, Case CL (2004). Microbiology: an introduction (8th ed.). San Francisco: Benjamin Cummings. ISBN 0-8053-7614-3.